이상인산칼슘 스캐폴드의 3차원적으로 상호 연결된 미세 기공이 골아세포거동에 미치는 영향
The effect of interconnective micropores of biphasic calcium phosphate scaffold on osteoblast cellular behavior
이문환, 유창국, 이효진, 권태엽, 김교한,
소속 상세정보
이문환 ( Lee Mun-Hwan ) - 경북대학교 대학원 의용생체공학과
유창국 ( You Chang-Kook ) - 경북대학교 대학원 의용생체공학과
이효진 ( Lee Hyo-Jin ) - 경북대학교 치의학전문대학원 치과생체재료학교실
권태엽 ( Kwon Tae-Yub ) - 경북대학교 대학원 의용생체공학과
김교한 ( Kim Kho-Han ) - 경북대학교 대학원 의용생체공학과
KMID : 0362220130400040359
Abstract
The objective of this study was to evaluate the effect of interconnective micropore on osteoblast proliferation and spreading. The micropores of scaffold were observed by field emission scanning electron microscope. Surface roughness of slurry coated surface was determined by the atomic force microscope. Contact angle goniometer was used to evaluate differences in contact angle between surfaces. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay was used to measure the cell proliferation. The surface roughness of microporous specimens was higher than that of non-microporous specimen. The contact angles of microporous specimens were lower than that of non-microporous specimen. Cell spread was greater on microporous scaffolds than non-microporous scaffolds. In addition, cell proliferation was increased when cells were cultured on microporous scaffold. The result demonstrated that interconnective micropores of biphasic calcium phosphate scaffold generally improved the response of osteoblast in-vitro. This behavior could be explained by surface roughness and hydrophilicity.
키워드
Interconnective micropores; Biphasic calcium phosphate scaffold; Roughness; Contact angle; Proliferation; Spreading
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